Figure 3.

MP does not affect CD4⁺ cell proportion or apoptosis rate among CNS-infiltrating cells. SCC were isolated from DA rats immunized with SCH-CFA, and injected with MP or with PBS, as described in Methods. SCC were stained with anti-CD4 and anti-CD11b specific antibodies (A), with anti-CD4 and anti-CD62L or anti-OX40 or anti-CD25 specific antibodies (B), or with anti-CD4 specific antibody and Annexin V (C). Alternatively, SCC from PBS-treated (D) and MP-treated (E) rats were stained with anti-CD4 antibody and intracellular staining for IFN-γ and IL-17 was performed. The results presented in B-E are gated to CD4⁺ cells. Results of intracellular staining (D, E) are representative of three independent experiments. *p < 0.05 represents a statistically significant difference between the MP and PBS groups.