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Gene expression in A-myb null mice. (A). Western blot of three organs from wild type CD-1 mice and from testes of 129/Jx C57BL/6 A-myb null and wt littermates. Top, anti RFX2. Bottom, anti SP1. (B). Determination of mRNA for Rfx1-4 in testes of A-myb null and wt littermates by quantitative real-time PCR. (C). Determination of mRNAs for reference genes c-Kit, H1t, and Ldhc in testes of A-myb null and wt littermates by quantitative real-time PCR.
