Table 1.
Primers used in this study
| Purpose | Primer sequence (5'-3') | |
|---|---|---|
| Forward | Reverse | |
| NS1 pSos |
CGGTCGACGATGGATTCCAACACTGTGT | ACGTGCGGCCGC ATCAGCCATCTTATCTCTTC |
| NS1 pCMV-Myc |
ACGCGTCGACCATGGATTCCAACACTGTGTC | TTGCGGCCGC TCAATCAGCCATCTTATCTC |
| NS1 pEGFP-N3 |
CCGAATTCTATGGATTCCAACACTGTGTC | GGGGTACC ATCAGCCATCTTATCTCTTC |
| NS1 pGEX-6P-1 |
CCGAATTCATGGATTCCAACACTGTGTC | TTGCGGCCGC TCAATCAGCCATCTTATCTC |
| NS11-80 pCMV-Myc |
ACGCGTCGACC ATGGATTCCAACACTGTGTC | TTGCGGCCGC TCAGGTCATTGTAAGCGCCTC |
| NS181-238 pCMV-Myc |
ACGCGTCGACC ATGGCCTCCACACCTGC | TTGCGGCCGC TCAATCAGCCATCTTATCTC |
| GAS8 pDsRed-Express-C1 | GGAATTCGATGGCACCGAAAAAGAAAGGGAAGA | GGGGTACCCGTCGGGGTGCCCACCAGTCCCGC |
| GAS8 pCMV-Tag 2B |
CGGAATTCATGGCACCGAAAAAGAAAGGGAAGA | CCCTCGAGTTACGTCGGGGTGCCCACCAGTCC |
| GAS81-260 pCMV-Tag 2B |
CGGAATTCATGGCACCGAAAAAGAAAGGGAAGA | CCCTCGAGTTACTTCCGCATGTCCTCCATCTG |
| GAS8260-478 pCMV-Tag 2B |
CGGAATTCAAGGAGGACCACCTGGAGAGGG | CCCTCGAGTTACGTCGGGGTGCCCACCAGTCCCGC |