Table 2.
Primers and probes for TaqMan qRT-PCR
| Gene Sequence (5'-> 3') | Amplicon (bp) | Genebank Acc.No |
|---|---|---|
| PDGFA | ||
| (+) TCGATGAGATGGAGGGTCG | 71 | NM_033023 |
| (-) ACCCGGACAGAAATCCAGTCT | ||
| FAM-TGAGACCTCTGCACTTCCATCCCACG-TAMRA | ||
| PDGFB | ||
| Applied Biosystems Assay On Demand, Hs00234042_m1 | 80 | NM_033016.1 |
| PDGFC | ||
| (+) TATCTGATGAATATTTTCCTTCTGAACCAG | 96 | NM_016205 |
| (-) GTAGCACTGAAGGACTCACAGCTTCT | ||
| FAM-TTCTGCATCCACTACAACATTGTCATGCCACAATTC-TAMRA | ||
| PDGFD | ||
| Applied Biosystems Assay On Demand, Hs00228671_m1 | 73 | NM_033135.3 |
| PDGFRA | ||
| (+) CAAGAGGAACAGACACAGCTCGCAGA | 84 | NM_006206 |
| (-) CTGCTGGAACCCGTCTCAAT | ||
| FAM - CAAGAGGAACAGACACAGCTCGCAGA-TAMRA | ||
| PDGFRB | ||
| Applied Biosystems Assay On Demand, Hs00182163_m1 | 86 | NM_002609.3 |
| LTB2 | ||
| Applied Biosystems Assay On Demand, Hs00242739_m1 | 78 | NM_002341.1 |
| IL2RG | ||
| Applied Biosystems Assay On Demand, Hs00173950_m1 | 76 | NM_000206.1 |
| FYB | ||
| Applied Biosystems Assay On Demand, Hs01061565_m1 | 123 | NM_001465.3 |
| IL32 | ||
| Applied Biosystems Assay On Demand, Hs00992441_m1 | 85 | NM_004221.4 |
Individual assays were either designed (PDGFA, PDGFC and PDGFRA) or ordered from Applied Biosystems, Assay On Demand, (PDGFB, PDGFD, PDGFRB, LTB2, IL2RG, FYB and IL32).