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Higher levels of miRNAs are observed in the female Ae. aegypti midgut 24 hrs after blood feeding (Gut_BF) compared to sugar feeding (Gut_SF). Three-day old females were either fed on blood or sugar and dissected 24 hrs later. 10 μg of total RNA were used per sample. The top panels are northern results and the bottom panels are RNA gel images for verification of small ribosomal RNA and tRNA integrity and loading of total RNA.
