Table 1.
Name1, 2 | mirBase Name | Sequence3 | Contig4 | Start4 | End4 | Strand | Embryo5 | Gut_SF5 | Gut_BF5 |
---|---|---|---|---|---|---|---|---|---|
aae-miR-M1-1 | aae-miR-2943-1 | TTAAGTAGGCACTTGCAGGCAAA | CONTIG_15246 | 25749 | 25829 | + | 28 | 0 | 0 |
aae-miR-M1-2 | aae-miR-2943-2 | TTAAGTAGGCACTTGCAGGCAAA | CONTIG_15246 | 25911 | 25981 | + | 28 | 0 | 0 |
aae-miR-M2 | aae-miR-2942 | TATTCGAGACTTCACGAGTTAAT | CONTIG_11944 | 151669 | 151750 | + | 0 | 0 | 7 |
aae-miR-M3 | aae-miR-2945 | TGACTAGAGGCAGACTCGTTTA | CONTIG_2929 | 123975 | 124056 | + | 33 | 0 | 6 |
aae-miR-M3* | aae-miR-2945* | AGCGGGTCCGTTTCTAGTGTCATG | CONTIG_2929 | 123975 | 124056 | + | 3 | 0 | 0 |
aae-miR-M4a | aae-miR-2944a | GAAGGAACTTCTGCTGTGATCTGA | CONTIG_18252 | 93868 | 93929 | + | 91 | 2 | 0 |
aae-miR-M4a* | aae-miR-2944a* | TATCACAGTAGTTGTACTTTAA | CONTIG_18252 | 93868 | 93929 | + | 3 | 0 | 0 |
aae-miR-M4b | aae-miR-2944b | GAAGGAACTCCCGGTGTGATATA | CONTIG_18252 | 93731 | 93792 | + | 62 | 0 | 0 |
aae-miR-M4b* | aae-miR-2944b* | TATCACAGCAGTAGTTACCTGA | CONTIG_18252 | 93731 | 93792 | + | 24 | 0 | 0 |
aae-miR-N1-1 | aae-miR-2941-2 | TAGTACGGCTAGAACTCCACGGA | CONTIG_16241 | 318005 | 318105 | - | 288 | 1 | 1 |
aae-miR-N1-2 | aae-miR-2941-1 | TAGTACGGCTAGAACTCCACGGA | CONTIG_16241 | 317700 | 317799 | - | 288 | 1 | 1 |
aae-miR-N2 | aae-miR-2946 | TAGTACGGAAAAGATATGGGGA | CONTIG_16241 | 318135 | 318222 | - | 5 | 0 | 0 |
aae-miR-1174 | aae-miR-1174 | TCAGATCTACTTAATACCCAT | CONTIG_7232 | 17531 | 17665 | + | |||
aae-miR-1175 | aae-miR-1175 | TGAGATTCTACTTCTCCGACTTAA | CONTIG_7232 | 17737 | 17816 | + | 0 | 0 | 1 |
aae-miR-1175* | aae-miR-1175* | TAAGTGGAGTAGTGGTCTCATCGCT | CONTIG_7232 | 17737 | 17816 | + | 0 | 2 | 59 |
aae-miR-1890 | aae-miR-1890 | TGAAATCTTTGATTAGGTCTGG | CONTIG_10435 | 60786 | 60931 | + | 1 | 0 | 0 |
aae-miR-1891-1 | aae-miR-1891-1 | TGAGGAGTTAATTTGCGTGTTT | CONTIG_10244 | 3984 | 4069 | - | 2 | 0 | 0 |
aae-miR-1891-2 | aae-miR-1891-2 | TGAGGAGTTAATTTGCGTGTTT | CONTIG_18287 | 4656 | 4741 | + | 2 | 0 | 0 |
aae-miR-1889 | aae-miR-1889 | CACGTTACAGATTGGGGTTTCC | CONTIG_4323 | 23786 | 23921 | - | 0 | 0 | 3 |
aae-bantam | aae-bantam | TGAGATCATTTTGAAAGCTGATT | CONTIG_3301 | 208288 | 208426 | - | 1 | 2 | 2 |
aae-let-7 | aae-let-7 | TGAGGTAGTTGGTTGTATAGT | CONTIG_2929 | 321614 | 321684 | + | 0 | 0 | 5 |
aae-miR-1 | aae-miR-1 | TGGAATGTAAAGAAGTATGGAG | CONTIG_25061 | 66256 | 66338 | + | 1 | 0 | 1 |
aae-miR-10 | aae-miR-10 | ACCCTGTAGATCCGAATTTGTT | CONTIG_17639 | 8749 | 8833 | + | 1 | 0 | 0 |
aae-miR-100 | aae-miR-100 | AACCCGTAGATCCGAACTTGTG | CONTIG_2929 | 307441 | 307568 | + | |||
aae-miR-1000-1 | aae-miR-1000-1 | ATATTGTCCTGTCACAGCAGT | CONTIG_35246 | 172 | 265 | - | |||
aae-miR-1000-2 | aae-miR-1000-2 | ATATTGTCCTGTCACAGCAGT | CONTIG_9774 | 12985 | 13078 | + | |||
aae-miR-11 | aae-miR-11 | CATCACAGTCTGAGTTCTTGCTT | CONTIG_23911 | 46399 | 46488 | + | 1861 | 42 | 436 |
aae-miR-11* | aae-miR-11* | CGAGAACTCCGGCTGTGACCTGTG | CONTIG_23911 | 46399 | 46488 | + | 4 | 0 | 2 |
aae-miR-12 | aae-miR-12 | TGAGTATTACATCAGGTACTGGT | CONTIG_4323 | 23386 | 23503 | - | |||
aae-miR-124 | aae-miR-124 | TAAGGCACGCGGTGAATGCCAAG | CONTIG_531 | 21343 | 21423 | - | 3 | 0 | 0 |
aae-miR-125 | aae-miR-125 | TCCCTGAGACCCTAACTTGTGAC | CONTIG_2929 | 321885 | 321975 | + | 0 | 0 | 3 |
aae-miR-133 | aae-miR-133 | TTGGTCCCCTTCAACCAGCTGT | CONTIG_24522 | 50243 | 50347 | - | |||
aae-miR-137-1 | aae-miR-137-1 | TTATTGCTTGAGAATACACGTA | CONTIG_7773 | 15414 | 15486 | - | |||
aae-miR-137-2 | aae-miR-137-2 | TTATTGCTTGAGAATACACGTA | CONTIG_29705 | 92457 | 92529 | + | |||
aae-miR-13b | aae-miR-13 | TATCACAGCCATTTTGACGAGTT | CONTIG_12793 | 17619 | 17710 | - | 168 | 2 | 42 |
aae-miR-14 | aae-miR-14 | TCAGTCTTTTTCTCTCTCCTAT | CONTIG_12112 | 693 | 785 | - | 1 | 1 | 6 |
aae-miR-184 | aae-miR-184 | TGGACGGAGAACTGATAAGGGC | CONTIG_19030 | 3452 | 3535 | - | 957 | 76 | 1307 |
aae-miR-190 | aae-miR-190 | AGATATGTTTGATATTCTTGGTTG | CONTIG_10108 | 2092 | 2180 | - | 83 | 2 | 43 |
aae-miR-193 | aae-miR-193 | TACTGGCCTACTAAGTCCCAAC | CONTIG_19568 | 51035 | 51158 | + | |||
aae-miR-2a | aae-miR-2a | TATCACAGCCAGCTTTGATGAGCT | CONTIG_12793 | 16130 | 16216 | - | 1217 | 80 | 249 |
aae-miR-210 | aae-miR-210 | TTGTGCGTGTGACAACGGCTAT | CONTIG_19443 | 85221 | 85292 | - | 3 | 12 | 12 |
aae-miR-219 | aae-miR-219 | TGATTGTCCAAACGCAATTCTTG | CONTIG_15776 | 56993 | 57078 | + | |||
aae-miR-2b | aae-miR-2b | TATCACAGCCAGCTTTGAAGAGCG | CONTIG_12793 | 17471 | 17562 | - | 758 | 74 | 147 |
aae-miR-2c | aae-miR-2c | TATCACAGCCAGCTTTGATGAGC | CONTIG_12793 | 17971 | 18048 | - | 1218 | 80 | 249 |
aae-miR-252 | aae-miR-252 | CTAAGTACTAGTGCCGCAGGAGA | CONTIG_3685 | 79002 | 79177 | - | 38 | 0 | 1 |
aae-miR-252* | aae-miR-252* | CCTGCTGCCCAAGTGCTTATCGAA | CONTIG_3685 | 79002 | 79177 | - | 5 | 0 | 0 |
aae-miR-263 | aae-miR-263a | AATGGCACTGGAAGAATTCACGGG | CONTIG_27430 | 4746 | 4821 | - | 53 | 1 | 1 |
aae-miR-263* | aae-miR-263a* | CGTGTTCTGGCAGTGGCATCCC | CONTIG_27430 | 4746 | 4821 | - | 6 | 0 | 0 |
aae-miR-263b | aae-miR-263b | CTTGGCACTGGGAGAATTCACAG | CONTIG_1908 | 33267 | 33357 | + | 6 | 0 | 0 |
aae-miR-263b* | aae-miR-263b* | TGGATCTTTTCGTGCCATCGT | CONTIG_1908 | 33267 | 33357 | + | 1 | 0 | 0 |
aae-miR-275 | aae-miR-275 | TCAGGTACCTGAAGTAGCGCGCG | CONTIG_1651 | 96625 | 96720 | + | 0 | 0 | 5 |
aae-miR-275* | aae-miR-275* | CGCGCTAAGCAGGAACCGAGACT | CONTIG_1651 | 96625 | 96720 | + | 1 | 0 | 6 |
aae-miR-276-1 | aae-miR-276-1 | TAGGAACTTCATACCGTGCTCT | CONTIG_417 | 10988 | 11177 | - | |||
aae-miR-276-2 | aae-miR-276-2 | TAGGAACTTCATACCGTGCTCT | CONTIG_7617 | 38953 | 39087 | + | |||
aae-miR-277 | aae-miR-277 | TAAATGCACTATCTGGTACGACA | CONTIG_12646 | 3556 | 3644 | + | |||
aae-miR-278 | aae-miR-278 | TCGGTGGGACTTTCGTCCGTTT | CONTIG_1172 | 33037 | 33128 | + | 6 | 0 | 2 |
aae-miR-279 | aae-miR-279 | TGACTAGATCCACACTCATTAA | CONTIG_17556 | 116934 | 117006 | - | 26 | 0 | 2 |
aae-miR-281 | aae-miR-281 | CTGTCATGGAATTGCTCTCTTTA | CONTIG_27100 | 34036 | 34204 | + | 1 | 16 | 50 |
aae-miR-281* | aae-miR-281* | AAAGAGAGCTATCCGTCGACAGTA | CONTIG_27100 | 34036 | 34204 | + | 164 | 5686 | 4806 |
aae-miR-282-1 | aae-miR-282-1 | AATCTAGCCTCTCCTAGGCTTTGTCTG | CONTIG_15805 | 19414 | 19548 | + | |||
aae-miR-282-1* | aae-miR-282-1* | ACATAGCCTGACAGAGGTTAGG | CONTIG_15805 | 19414 | 19548 | + | 2 | 0 | 0 |
aae-miR-282-2 | aae-miR-282-2 | AATCTAGCCTCTCCTAGGCTTTGTCTG | CONTIG_15982 | 5479 | 5613 | - | |||
aae-miR-282-2* | aae-miR-282-2* | ACATAGCCTGACAGAGGTTAGG | CONTIG_15982 | 5479 | 5613 | - | 2 | 0 | 0 |
aae-miR-283 | aae-miR-283 | CAATATCAGCTGGTAATTCTGGG | CONTIG_4323 | 32334 | 32426 | - | 6 | 1 | 131 |
aae-miR-285 | aae-miR-285 | TAGCACCATTCGAAATCAGT | CONTIG_1634 | 212427 | 212492 | - | 0 | 1 | 0 |
aae-miR-286a-1 | aae-miR-286b-1 | TGACTAGACCGAACACTCGTATCC | CONTIG_21241 | 18407 | 18503 | + | 8 | 0 | 0 |
aae-miR-286a-2 | aae-miR-286b-2 | TGACTAGACCGAACACTCGTATCC | CONTIG_8291 | 17656 | 17752 | + | 8 | 0 | 0 |
aae-miR-286b | aae-miR-286a | TGACTAGACCGAACACTCGCGTCCT | CONTIG_18252 | 93413 | 93509 | + | 10 | 0 | 0 |
aae-miR-305 | aae-miR-305 | ATTGTACTTCATCAGGTGCTCTGG | CONTIG_1651 | 105677 | 105766 | + | 0 | 0 | 2 |
aae-miR-305* | aae-miR-305* | CGGCACATGTTGGAGTACACTTAA | CONTIG_1651 | 105677 | 105766 | + | 0 | 0 | 7 |
aae-miR-306 | aae-miR-306 | TCAGGTACTGAGTGACTCTCAG | CONTIG_24640 | 55004 | 55128 | + | 119 | 0 | 27 |
aae-miR-307 | aae-miR-307 | TCACAACCTCCTTGAGTGAGCGA | CONTIG_1157 | 147943 | 148043 | - | 1 | 0 | 0 |
aae-miR-308 | aae-miR-308 | AATCACAGGAGTATACTGTGAG | CONTIG_6324 | 6462 | 6528 | + | |||
aae-miR-308* | aae-miR-308* | CGCGGTATATTCTTGTGGCTTGA | CONTIG_6324 | 6462 | 6528 | + | 2 | 0 | 0 |
aae-miR-31 | aae-miR-31 | TGGCAAGATGTTGGCATAGCTGAAA | CONTIG_21960 | 98153 | 98301 | - | 1 | 0 | 3 |
aae-miR-315 | aae-miR-315 | TTTTGATTGTTGCTCAGAAAGCC | CONTIG_21501 | 12498 | 12562 | + | 27 | 1 | 0 |
aae-miR-315* | aae-miR-315* | CTTTCGAGCAGTAATCAAAGTc | CONTIG_21501 | 12498 | 12562 | + | 5 | 0 | 0 |
aae-miR-316 | aae-miR-316 | TGTCTTTTTCCGCTTACTGCCG | CONTIG_13460 | 10738 | 10828 | - | 0 | 0 | 1 |
aae-miR-317-1 | aae-miR-317-1 | TGAACACAGCTGGTGGTATCTCAGT | CONTIG_12640 | 88437 | 88524 | + | 36 | 38 | 737 |
aae-miR-317-2 | aae-miR-317-2 | TGAACACAGCTGGTGGTATCTCAGT | CONTIG_8451 | 9550 | 9637 | - | 36 | 38 | 737 |
aae-miR-3a-1 | aae-miR-309a-1 | TCACTGGGCAAAGTTTGTCGCA | CONTIG_21241 | 18967 | 19043 | + | 6 | 0 | 0 |
aae-miR-3a-2 | aae-miR-309a-2 | TCACTGGGCAAAGTTTGTCGCA | CONTIG_8291 | 18216 | 18292 | + | 6 | 0 | 0 |
aae-miR-3b | aaw-miR-309b | TCACTGGGCATAGTTTGTCGCA | CONTIG_18252 | 94047 | 94117 | + | 3 | 0 | 0 |
aae-miR-3b* | aae-miR-309b* | CGTCAAACTCCGTTCAGTTGGTG | CONTIG_18252 | 94047 | 94117 | + | 1 | 0 | 0 |
aae-miR-33 | aae-miR-33 | GTGCATTGTAGTTGCATTGCA | CONTIG_18815 | 108787 | 108866 | + | |||
aae-miR-34 | aae-miR-34 | TGGCAGTGTGGTTAGCTGGTTGTG | CONTIG_12646 | 4278 | 4400 | + | 44 | 55 | 639 |
aae-miR-375 | aae-miR-375 | TTTGTTCGTTTGGCTCGAGTTA | CONTIG_14081 | 238834 | 238944 | - | 1 | 0 | 0 |
aae-miR-7 | aae-miR-7 | TGGAAGACTAGTGATTTTGTTGTT | CONTIG_31115 | 46028 | 46112 | + | 14 | 0 | 0 |
aae-miR-71 | aae-miR-71 | AGAAAGACATGGGTAGTGAGATA | CONTIG_12793 | 18215 | 18396 | - | 39 | 2 | 7 |
aae-miR-71* | aae-miR-71* | TCTCACTACCTTGTCTTTCATG | CONTIG_12793 | 18215 | 18396 | - | 6 | 0 | 0 |
aae-miR-79 | aae-miR-79 | ATAAAGCTAGATTACCAAAGCAT | CONTIG_24640 | 55215 | 55284 | + | 2 | 0 | 0 |
aae-miR-8 | aae-miR-8 | TAATACTGTCAGGTAAAGATGTC | CONTIG_16942 | 34594 | 34660 | + | 32 | 1 | 38 |
aae-miR-8* | aae-miR-8* | CATCTTACCGGGCAGCATTAGA | CONTIG_16942 | 34594 | 34660 | + | 7 | 2 | 4 |
aae-miR-87 | aae-miR-87 | GGTGAGCAAATTTTCAGGTGT | CONTIG_15587 | 30157 | 30251 | + | |||
aae-miR-927 | aae-miR-927 | TTTAGAATTCCTACGCTTTACC | CONTIG_1795 | 170460 | 170534 | + | |||
aae-miR-929-1 | aae-miR-929-1 | ATTGACTCTAGTAGGGAGTCC | CONTIG_12461 | 76647 | 76734 | - | |||
aae-miR-929-2 | aae-miR-929-2 | ATTGACTCTAGTAGGGAGTCC | CONTIG_34315 | 3294 | 3381 | + | |||
aae-miR-92a | aae-miR-92a | TATTGCACTTGTCCCGGCCTAT | CONTIG_6821 | 67755 | 67832 | + | 74 | 0 | 5 |
aae-miR-92a* | aae-miR-92a* | CGGTACGGACAGGGGCAACATT | CONTIG_6821 | 67755 | 67832 | + | 6 | 0 | 0 |
aae-miR-92b | aae-miR-92b | AATTGCACTTGTCCCGGCCTGC | CONTIG_6824 | 4049 | 4131 | + | 74 | 0 | 5 |
aae-miR-92b* | aae-miR-92b* | AGGTCGTGACTTGTGCCCGTTTG | CONTIG_6824 | 4049 | 4131 | + | 12 | 0 | 0 |
aae-miR-932 | aae-miR-932 | TCAATTCCGTAGTGCATTGCAG | CONTIG_28378 | 72232 | 72319 | - | |||
aae-miR-957 | aae-miR-957 | TGAAACCGTCCAAAACTGAGGC | CONTIG_591 | 147619 | 147787 | + | |||
aae-miR-965 | aae-miR-965 | TAAGCGTATAGCTTTTCCC | CONTIG_3410 | 87378 | 87524 | + | |||
aae-miR-970 | aae-miR-970 | TCATAAGACACACGCGGCTAT | CONTIG_11324 | 29252 | 29338 | + | 1 | 0 | 3 |
aae-miR-981 | aae-miR-981 | TTCGTTGTCGACGAAACCTGCA | CONTIG_7302 | 184768 | 184853 | - | |||
aae-miR-988 | aae-miR-988 | CCCCTTGTTGCAAACCTCACGC | CONTIG_17684 | 77966 | 78086 | - | 3 | 0 | 1 |
aae-miR-988* | aae-miR-988* | GTGTGCTTTGTGACAATGAGA | CONTIG_17684 | 77966 | 78086 | - | 2 | 0 | 2 |
aae-miR-989 | aae-miR-989 | TGTGATGTGACGTAGTGGTAC | CONTIG_6774 | 51048 | 51137 | - | 2 | 33 | 3 |
aae-miR-993 | aae-miR-993 | GAAGCTCGTTTCTATAGAGGTATCT | CONTIG_28305 | 82579 | 82660 | + | |||
aae-miR-996 | aae-miR-996 | TGACTAGATTACATGCTCGTCT | CONTIG_17556 | 112174 | 112267 | - | |||
aae-miR-998 | aae-miR-998 | TAGCACCATGAGATTCAGCTC | CONTIG_23911 | 46676 | 46764 | + | 164 | 6 | 42 |
aae-miR-999 | aae-miR-999 | TGTTAACTGTAAGACTGTGTCT | CONTIG_6027 | 163128 | 163269 | + | |||
aae-miR-9a-1 | aae-miR-9a-1 | TCTTTGGTTATCTAGCTGTATGA | CONTIG_19539 | 116871 | 116951 | + | 16 | 1 | 2 |
aae-miR-9a-2 | aae-miR-9a-2 | TCTTTGGTTATCTAGCTGTATGA | CONTIG_19541 | 4061 | 4141 | + | 16 | 1 | 2 |
aae-miR-9b | aae-miR-9b | TCTTTGGTGATTTTAGCTGTATGC | CONTIG_24640 | 55513 | 55604 | + | 134 | 0 | 69 |
aae-miR-9c | aae-miR-9c | TCTAAAGCTTTAGTACCAGAGGTC | CONTIG_24640 | 28160 | 28257 | + | 5 | 0 | 0 |
aae-miR-iab-4-1 | aae-miR-iab-4-1 | CGGTATACCTTCAGTATACGTAAC | CONTIG_17255 | 44653 | 44726 | - | |||
aae-miR-iab-4-2 | aae-miR-iab-4-2 | CGGTATACCTTCAGTATACGTAAC | CONTIG_23219 | 23581 | 23654 | + |
Notes:
1. The first block (miR-M1-1 through miR-N2) are novel miRNAs that are discovered in this study. We have not detected homology to any known miRNAs or genomic sequences outside of mosquito species. The second block (miR-1174 through miR-1889) contains miRNAs that are homologous to previously reported "mosquito-specific" miRNAs. The third block, comprised of the remaining miRNAs, contains miRNAs that have homologues outside of mosquitoes.
2. Naming in this column is temporary. Mirbase assigned names are shown in column 2, which was received after the acceptance of the manuscript. "-1", and "-2" suffixes refer to different hairpins that produce the same mature miRNA. "a", and "b" suffixes refer to different hairpins that produce similar but not identical miRNAs.
3. Underlined sequences are cases where there are extra bases at the 5' or 3' ends and they are the majority in at least six small RNA sequences. Italicized sequences are cases where such sequences are detected but they are either not the majority or there are less than six 454 sequence hits. As poly-As are added to the small RNAs, adenine(s) at the 3' end cannot be confirmed by this sequencing approach.
4. The contig, start, and end positions refer to the locations of the pre-miRNA hairpins.
5. The last three columns are the number of sequence hits in small RNA libraries obtained by 454 sequencing. The total small RNA reads are 55, 000, 33, 000, and 42, 000 in embryos, sugar-fed midguts (Gut_SF), and blood-fed midguts (Gut_BF), respectively. The total number of hits for all Ae. aegypti miRNAs are 8369, 6260, and 9922 in embryos, sugar-fed midguts (Gut_SF), and blood-fed midguts (Gut_BF), respectively. There are a few cases where we did not distinguish the hits from nearly identical miRNAs.