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. 2009 Dec 4;10:581. doi: 10.1186/1471-2164-10-581

Table 1.

Sequence, location, and expression of miRNAs in Aedes aegypti.

Name1, 2 mirBase Name Sequence3 Contig4 Start4 End4 Strand Embryo5 Gut_SF5 Gut_BF5
aae-miR-M1-1 aae-miR-2943-1 TTAAGTAGGCACTTGCAGGCAAA CONTIG_15246 25749 25829 + 28 0 0
aae-miR-M1-2 aae-miR-2943-2 TTAAGTAGGCACTTGCAGGCAAA CONTIG_15246 25911 25981 + 28 0 0
aae-miR-M2 aae-miR-2942 TATTCGAGACTTCACGAGTTAAT CONTIG_11944 151669 151750 + 0 0 7
aae-miR-M3 aae-miR-2945 TGACTAGAGGCAGACTCGTTTA CONTIG_2929 123975 124056 + 33 0 6
aae-miR-M3* aae-miR-2945* AGCGGGTCCGTTTCTAGTGTCATG CONTIG_2929 123975 124056 + 3 0 0
aae-miR-M4a aae-miR-2944a GAAGGAACTTCTGCTGTGATCTGA CONTIG_18252 93868 93929 + 91 2 0
aae-miR-M4a* aae-miR-2944a* TATCACAGTAGTTGTACTTTAA CONTIG_18252 93868 93929 + 3 0 0
aae-miR-M4b aae-miR-2944b GAAGGAACTCCCGGTGTGATATA CONTIG_18252 93731 93792 + 62 0 0
aae-miR-M4b* aae-miR-2944b* TATCACAGCAGTAGTTACCTGA CONTIG_18252 93731 93792 + 24 0 0
aae-miR-N1-1 aae-miR-2941-2 TAGTACGGCTAGAACTCCACGGA CONTIG_16241 318005 318105 - 288 1 1
aae-miR-N1-2 aae-miR-2941-1 TAGTACGGCTAGAACTCCACGGA CONTIG_16241 317700 317799 - 288 1 1
aae-miR-N2 aae-miR-2946 TAGTACGGAAAAGATATGGGGA CONTIG_16241 318135 318222 - 5 0 0
aae-miR-1174 aae-miR-1174 TCAGATCTACTTAATACCCAT CONTIG_7232 17531 17665 +
aae-miR-1175 aae-miR-1175 TGAGATTCTACTTCTCCGACTTAA CONTIG_7232 17737 17816 + 0 0 1
aae-miR-1175* aae-miR-1175* TAAGTGGAGTAGTGGTCTCATCGCT CONTIG_7232 17737 17816 + 0 2 59
aae-miR-1890 aae-miR-1890 TGAAATCTTTGATTAGGTCTGG CONTIG_10435 60786 60931 + 1 0 0
aae-miR-1891-1 aae-miR-1891-1 TGAGGAGTTAATTTGCGTGTTT CONTIG_10244 3984 4069 - 2 0 0
aae-miR-1891-2 aae-miR-1891-2 TGAGGAGTTAATTTGCGTGTTT CONTIG_18287 4656 4741 + 2 0 0
aae-miR-1889 aae-miR-1889 CACGTTACAGATTGGGGTTTCC CONTIG_4323 23786 23921 - 0 0 3
aae-bantam aae-bantam TGAGATCATTTTGAAAGCTGATT CONTIG_3301 208288 208426 - 1 2 2
aae-let-7 aae-let-7 TGAGGTAGTTGGTTGTATAGT CONTIG_2929 321614 321684 + 0 0 5
aae-miR-1 aae-miR-1 TGGAATGTAAAGAAGTATGGAG CONTIG_25061 66256 66338 + 1 0 1
aae-miR-10 aae-miR-10 ACCCTGTAGATCCGAATTTGTT CONTIG_17639 8749 8833 + 1 0 0
aae-miR-100 aae-miR-100 AACCCGTAGATCCGAACTTGTG CONTIG_2929 307441 307568 +
aae-miR-1000-1 aae-miR-1000-1 ATATTGTCCTGTCACAGCAGT CONTIG_35246 172 265 -
aae-miR-1000-2 aae-miR-1000-2 ATATTGTCCTGTCACAGCAGT CONTIG_9774 12985 13078 +
aae-miR-11 aae-miR-11 CATCACAGTCTGAGTTCTTGCTT CONTIG_23911 46399 46488 + 1861 42 436
aae-miR-11* aae-miR-11* CGAGAACTCCGGCTGTGACCTGTG CONTIG_23911 46399 46488 + 4 0 2
aae-miR-12 aae-miR-12 TGAGTATTACATCAGGTACTGGT CONTIG_4323 23386 23503 -
aae-miR-124 aae-miR-124 TAAGGCACGCGGTGAATGCCAAG CONTIG_531 21343 21423 - 3 0 0
aae-miR-125 aae-miR-125 TCCCTGAGACCCTAACTTGTGAC CONTIG_2929 321885 321975 + 0 0 3
aae-miR-133 aae-miR-133 TTGGTCCCCTTCAACCAGCTGT CONTIG_24522 50243 50347 -
aae-miR-137-1 aae-miR-137-1 TTATTGCTTGAGAATACACGTA CONTIG_7773 15414 15486 -
aae-miR-137-2 aae-miR-137-2 TTATTGCTTGAGAATACACGTA CONTIG_29705 92457 92529 +
aae-miR-13b aae-miR-13 TATCACAGCCATTTTGACGAGTT CONTIG_12793 17619 17710 - 168 2 42
aae-miR-14 aae-miR-14 TCAGTCTTTTTCTCTCTCCTAT CONTIG_12112 693 785 - 1 1 6
aae-miR-184 aae-miR-184 TGGACGGAGAACTGATAAGGGC CONTIG_19030 3452 3535 - 957 76 1307
aae-miR-190 aae-miR-190 AGATATGTTTGATATTCTTGGTTG CONTIG_10108 2092 2180 - 83 2 43
aae-miR-193 aae-miR-193 TACTGGCCTACTAAGTCCCAAC CONTIG_19568 51035 51158 +
aae-miR-2a aae-miR-2a TATCACAGCCAGCTTTGATGAGCT CONTIG_12793 16130 16216 - 1217 80 249
aae-miR-210 aae-miR-210 TTGTGCGTGTGACAACGGCTAT CONTIG_19443 85221 85292 - 3 12 12
aae-miR-219 aae-miR-219 TGATTGTCCAAACGCAATTCTTG CONTIG_15776 56993 57078 +
aae-miR-2b aae-miR-2b TATCACAGCCAGCTTTGAAGAGCG CONTIG_12793 17471 17562 - 758 74 147
aae-miR-2c aae-miR-2c TATCACAGCCAGCTTTGATGAGC CONTIG_12793 17971 18048 - 1218 80 249
aae-miR-252 aae-miR-252 CTAAGTACTAGTGCCGCAGGAGA CONTIG_3685 79002 79177 - 38 0 1
aae-miR-252* aae-miR-252* CCTGCTGCCCAAGTGCTTATCGAA CONTIG_3685 79002 79177 - 5 0 0
aae-miR-263 aae-miR-263a AATGGCACTGGAAGAATTCACGGG CONTIG_27430 4746 4821 - 53 1 1
aae-miR-263* aae-miR-263a* CGTGTTCTGGCAGTGGCATCCC CONTIG_27430 4746 4821 - 6 0 0
aae-miR-263b aae-miR-263b CTTGGCACTGGGAGAATTCACAG CONTIG_1908 33267 33357 + 6 0 0
aae-miR-263b* aae-miR-263b* TGGATCTTTTCGTGCCATCGT CONTIG_1908 33267 33357 + 1 0 0
aae-miR-275 aae-miR-275 TCAGGTACCTGAAGTAGCGCGCG CONTIG_1651 96625 96720 + 0 0 5
aae-miR-275* aae-miR-275* CGCGCTAAGCAGGAACCGAGACT CONTIG_1651 96625 96720 + 1 0 6
aae-miR-276-1 aae-miR-276-1 TAGGAACTTCATACCGTGCTCT CONTIG_417 10988 11177 -
aae-miR-276-2 aae-miR-276-2 TAGGAACTTCATACCGTGCTCT CONTIG_7617 38953 39087 +
aae-miR-277 aae-miR-277 TAAATGCACTATCTGGTACGACA CONTIG_12646 3556 3644 +
aae-miR-278 aae-miR-278 TCGGTGGGACTTTCGTCCGTTT CONTIG_1172 33037 33128 + 6 0 2
aae-miR-279 aae-miR-279 TGACTAGATCCACACTCATTAA CONTIG_17556 116934 117006 - 26 0 2
aae-miR-281 aae-miR-281 CTGTCATGGAATTGCTCTCTTTA CONTIG_27100 34036 34204 + 1 16 50
aae-miR-281* aae-miR-281* AAAGAGAGCTATCCGTCGACAGTA CONTIG_27100 34036 34204 + 164 5686 4806
aae-miR-282-1 aae-miR-282-1 AATCTAGCCTCTCCTAGGCTTTGTCTG CONTIG_15805 19414 19548 +
aae-miR-282-1* aae-miR-282-1* ACATAGCCTGACAGAGGTTAGG CONTIG_15805 19414 19548 + 2 0 0
aae-miR-282-2 aae-miR-282-2 AATCTAGCCTCTCCTAGGCTTTGTCTG CONTIG_15982 5479 5613 -
aae-miR-282-2* aae-miR-282-2* ACATAGCCTGACAGAGGTTAGG CONTIG_15982 5479 5613 - 2 0 0
aae-miR-283 aae-miR-283 CAATATCAGCTGGTAATTCTGGG CONTIG_4323 32334 32426 - 6 1 131
aae-miR-285 aae-miR-285 TAGCACCATTCGAAATCAGT CONTIG_1634 212427 212492 - 0 1 0
aae-miR-286a-1 aae-miR-286b-1 TGACTAGACCGAACACTCGTATCC CONTIG_21241 18407 18503 + 8 0 0
aae-miR-286a-2 aae-miR-286b-2 TGACTAGACCGAACACTCGTATCC CONTIG_8291 17656 17752 + 8 0 0
aae-miR-286b aae-miR-286a TGACTAGACCGAACACTCGCGTCCT CONTIG_18252 93413 93509 + 10 0 0
aae-miR-305 aae-miR-305 ATTGTACTTCATCAGGTGCTCTGG CONTIG_1651 105677 105766 + 0 0 2
aae-miR-305* aae-miR-305* CGGCACATGTTGGAGTACACTTAA CONTIG_1651 105677 105766 + 0 0 7
aae-miR-306 aae-miR-306 TCAGGTACTGAGTGACTCTCAG CONTIG_24640 55004 55128 + 119 0 27
aae-miR-307 aae-miR-307 TCACAACCTCCTTGAGTGAGCGA CONTIG_1157 147943 148043 - 1 0 0
aae-miR-308 aae-miR-308 AATCACAGGAGTATACTGTGAG CONTIG_6324 6462 6528 +
aae-miR-308* aae-miR-308* CGCGGTATATTCTTGTGGCTTGA CONTIG_6324 6462 6528 + 2 0 0
aae-miR-31 aae-miR-31 TGGCAAGATGTTGGCATAGCTGAAA CONTIG_21960 98153 98301 - 1 0 3
aae-miR-315 aae-miR-315 TTTTGATTGTTGCTCAGAAAGCC CONTIG_21501 12498 12562 + 27 1 0
aae-miR-315* aae-miR-315* CTTTCGAGCAGTAATCAAAGTc CONTIG_21501 12498 12562 + 5 0 0
aae-miR-316 aae-miR-316 TGTCTTTTTCCGCTTACTGCCG CONTIG_13460 10738 10828 - 0 0 1
aae-miR-317-1 aae-miR-317-1 TGAACACAGCTGGTGGTATCTCAGT CONTIG_12640 88437 88524 + 36 38 737
aae-miR-317-2 aae-miR-317-2 TGAACACAGCTGGTGGTATCTCAGT CONTIG_8451 9550 9637 - 36 38 737
aae-miR-3a-1 aae-miR-309a-1 TCACTGGGCAAAGTTTGTCGCA CONTIG_21241 18967 19043 + 6 0 0
aae-miR-3a-2 aae-miR-309a-2 TCACTGGGCAAAGTTTGTCGCA CONTIG_8291 18216 18292 + 6 0 0
aae-miR-3b aaw-miR-309b TCACTGGGCATAGTTTGTCGCA CONTIG_18252 94047 94117 + 3 0 0
aae-miR-3b* aae-miR-309b* CGTCAAACTCCGTTCAGTTGGTG CONTIG_18252 94047 94117 + 1 0 0
aae-miR-33 aae-miR-33 GTGCATTGTAGTTGCATTGCA CONTIG_18815 108787 108866 +
aae-miR-34 aae-miR-34 TGGCAGTGTGGTTAGCTGGTTGTG CONTIG_12646 4278 4400 + 44 55 639
aae-miR-375 aae-miR-375 TTTGTTCGTTTGGCTCGAGTTA CONTIG_14081 238834 238944 - 1 0 0
aae-miR-7 aae-miR-7 TGGAAGACTAGTGATTTTGTTGTT CONTIG_31115 46028 46112 + 14 0 0
aae-miR-71 aae-miR-71 AGAAAGACATGGGTAGTGAGATA CONTIG_12793 18215 18396 - 39 2 7
aae-miR-71* aae-miR-71* TCTCACTACCTTGTCTTTCATG CONTIG_12793 18215 18396 - 6 0 0
aae-miR-79 aae-miR-79 ATAAAGCTAGATTACCAAAGCAT CONTIG_24640 55215 55284 + 2 0 0
aae-miR-8 aae-miR-8 TAATACTGTCAGGTAAAGATGTC CONTIG_16942 34594 34660 + 32 1 38
aae-miR-8* aae-miR-8* CATCTTACCGGGCAGCATTAGA CONTIG_16942 34594 34660 + 7 2 4
aae-miR-87 aae-miR-87 GGTGAGCAAATTTTCAGGTGT CONTIG_15587 30157 30251 +
aae-miR-927 aae-miR-927 TTTAGAATTCCTACGCTTTACC CONTIG_1795 170460 170534 +
aae-miR-929-1 aae-miR-929-1 ATTGACTCTAGTAGGGAGTCC CONTIG_12461 76647 76734 -
aae-miR-929-2 aae-miR-929-2 ATTGACTCTAGTAGGGAGTCC CONTIG_34315 3294 3381 +
aae-miR-92a aae-miR-92a TATTGCACTTGTCCCGGCCTAT CONTIG_6821 67755 67832 + 74 0 5
aae-miR-92a* aae-miR-92a* CGGTACGGACAGGGGCAACATT CONTIG_6821 67755 67832 + 6 0 0
aae-miR-92b aae-miR-92b AATTGCACTTGTCCCGGCCTGC CONTIG_6824 4049 4131 + 74 0 5
aae-miR-92b* aae-miR-92b* AGGTCGTGACTTGTGCCCGTTTG CONTIG_6824 4049 4131 + 12 0 0
aae-miR-932 aae-miR-932 TCAATTCCGTAGTGCATTGCAG CONTIG_28378 72232 72319 -
aae-miR-957 aae-miR-957 TGAAACCGTCCAAAACTGAGGC CONTIG_591 147619 147787 +
aae-miR-965 aae-miR-965 TAAGCGTATAGCTTTTCCC CONTIG_3410 87378 87524 +
aae-miR-970 aae-miR-970 TCATAAGACACACGCGGCTAT CONTIG_11324 29252 29338 + 1 0 3
aae-miR-981 aae-miR-981 TTCGTTGTCGACGAAACCTGCA CONTIG_7302 184768 184853 -
aae-miR-988 aae-miR-988 CCCCTTGTTGCAAACCTCACGC CONTIG_17684 77966 78086 - 3 0 1
aae-miR-988* aae-miR-988* GTGTGCTTTGTGACAATGAGA CONTIG_17684 77966 78086 - 2 0 2
aae-miR-989 aae-miR-989 TGTGATGTGACGTAGTGGTAC CONTIG_6774 51048 51137 - 2 33 3
aae-miR-993 aae-miR-993 GAAGCTCGTTTCTATAGAGGTATCT CONTIG_28305 82579 82660 +
aae-miR-996 aae-miR-996 TGACTAGATTACATGCTCGTCT CONTIG_17556 112174 112267 -
aae-miR-998 aae-miR-998 TAGCACCATGAGATTCAGCTC CONTIG_23911 46676 46764 + 164 6 42
aae-miR-999 aae-miR-999 TGTTAACTGTAAGACTGTGTCT CONTIG_6027 163128 163269 +
aae-miR-9a-1 aae-miR-9a-1 TCTTTGGTTATCTAGCTGTATGA CONTIG_19539 116871 116951 + 16 1 2
aae-miR-9a-2 aae-miR-9a-2 TCTTTGGTTATCTAGCTGTATGA CONTIG_19541 4061 4141 + 16 1 2
aae-miR-9b aae-miR-9b TCTTTGGTGATTTTAGCTGTATGC CONTIG_24640 55513 55604 + 134 0 69
aae-miR-9c aae-miR-9c TCTAAAGCTTTAGTACCAGAGGTC CONTIG_24640 28160 28257 + 5 0 0
aae-miR-iab-4-1 aae-miR-iab-4-1 CGGTATACCTTCAGTATACGTAAC CONTIG_17255 44653 44726 -
aae-miR-iab-4-2 aae-miR-iab-4-2 CGGTATACCTTCAGTATACGTAAC CONTIG_23219 23581 23654 +

Notes:

1. The first block (miR-M1-1 through miR-N2) are novel miRNAs that are discovered in this study. We have not detected homology to any known miRNAs or genomic sequences outside of mosquito species. The second block (miR-1174 through miR-1889) contains miRNAs that are homologous to previously reported "mosquito-specific" miRNAs. The third block, comprised of the remaining miRNAs, contains miRNAs that have homologues outside of mosquitoes.

2. Naming in this column is temporary. Mirbase assigned names are shown in column 2, which was received after the acceptance of the manuscript. "-1", and "-2" suffixes refer to different hairpins that produce the same mature miRNA. "a", and "b" suffixes refer to different hairpins that produce similar but not identical miRNAs.

3. Underlined sequences are cases where there are extra bases at the 5' or 3' ends and they are the majority in at least six small RNA sequences. Italicized sequences are cases where such sequences are detected but they are either not the majority or there are less than six 454 sequence hits. As poly-As are added to the small RNAs, adenine(s) at the 3' end cannot be confirmed by this sequencing approach.

4. The contig, start, and end positions refer to the locations of the pre-miRNA hairpins.

5. The last three columns are the number of sequence hits in small RNA libraries obtained by 454 sequencing. The total small RNA reads are 55, 000, 33, 000, and 42, 000 in embryos, sugar-fed midguts (Gut_SF), and blood-fed midguts (Gut_BF), respectively. The total number of hits for all Ae. aegypti miRNAs are 8369, 6260, and 9922 in embryos, sugar-fed midguts (Gut_SF), and blood-fed midguts (Gut_BF), respectively. There are a few cases where we did not distinguish the hits from nearly identical miRNAs.