Table 2.
Comparison of the number of miRNA sequences in sugar-fed and blood-fed midgut samples.
| miRNAs 1 | Gut_SF 6 | Gut_BF 6 | Fold Change I 7, 9 | Fold Change II 8, 9 |
|---|---|---|---|---|
| aae-miR-11 2 | 42 | 436 | 6.55 | 8.16 |
| aae-miR-1175* | 2 | 59 | 18.61 | 23.18 |
| aae-miR-13b 3 | 2 | 42 | 13.25 | 16.50 |
| aae-miR-184 | 76 | 1307 | 10.85 | 13.51 |
| aae-miR-190 | 2 | 43 | 13.56 | 16.89 |
| aae-miR-281 | 16 | 50 | 1.97 | 2.46 |
| aae-miR-281* | 5686 | 4806 | 0.53 | 0.66 |
| aae-miR-283 | 1 | 131 | 82.65 | 102.93 |
| aae-miR-2a/2b/2c 3, 4 | 80 | 249 | 1.96 | 2.45 |
| aae-miR-306 5 | 0 | 27 | NA 10 | NA 10 |
| aae-miR-317-1 | 38 | 737 | 12.24 | 15.24 |
| aae-miR-317-2 | 38 | 737 | 12.24 | 15.24 |
| aae-miR-34 | 55 | 639 | 7.33 | 9.13 |
| aae-miR-8 | 1 | 38 | 23.98 | 29.86 |
| aae-miR-989 | 33 | 3 | 0.06 | 0.07 |
| aae-miR-998 2 | 6 | 42 | 4.42 | 5.50 |
| aae-miR-9b 5 | 0 | 69 | NA 10 | NA 10 |
Notes.
1. Only miRNAs that showed 25 or more sequences in one of the gut samples are shown.
2. MiR-11 and miR-998 are physically linked, less than 300 bp apart.
3. MiR-13b and miR-2a/2b/2c are in a physically linked cluster. Mir-13b is less than 200 bp apart from miR-2b.
4. It is difficult to distinguish between hits that match miR-2a, miR-2b, or miR-2c. Thus we refer to these hits miR-2a/2b/2c.
5. MiR-306 and miR-9b are physically linked, less than 500 bp apart.
6. The second and third columns are raw numbers of sequence hits in sugar-fed (Gut_SF) and blood-fed (Gut_BF) midgut samples, respectively.
7. Column four is the fold difference of Gut_BF over Gut_SF, normalized by the total miRNA hits within a sample, as suggested in reference [12]. The total miRNA hits are 6260 and 9922 in Gut_SF and Gut_BF samples, respectively. Thus, for each miRNA, the Fold Change = [(Raw Gut_BF number)/9922]/[(Raw Gut_SF number)/6260].
8. Column five is the fold difference of Gut_BF over Gut_SF, normalized by the total small RNA reads within a sample. The total small RNA reads are 33, 000 and 42, 000 in Gut_SF and Gut_BF samples, respectively. Thus, for each miRNA, the Fold Change = [(Raw Gut_BF number)/42000]/[(Raw Gut_SF number)/33000].
9. The numbers in columns 4 and 5 reflect the same trend, generally higher levels of these miRNAs in blood-fed sample than in sugar-fed sample. Although the number of small RNA sequences may not be sufficient for the analysis to be quantitative, overall trends suggest leads for further analysis.
10. NA, not applicable as the denominator is zero.