Fig. 1.

Purification of capsicein from P. capsici Pa28 and the induction of a hypersensitive response by CAP-Pa28. a Elution of capsicein from a Superdex 200 HR gel filtration column. b SDS–PAGE of capsicein, purified from culture filtrate. M, molecular size markers; 1, dialyzed culture filtrate after 95% ammonium sulfate precipitation; 2, after filtering through 0.2 μm pore size membrane (Whatman); 3, CAP-Pa28 fraction from FPLC gel filtration. c The hypersensitive response induced in Brassica rapa subsp. pekinensis and Nicotiana glutinosa leaves by 50 nmol of purified CAP-Pa28