Fig. 3.
Purification and the hypersensitive-response activity of recombinant capsicein GST–Cap-Pa28. a SDS–PAGE analysis of GST–Cap-Pa28. M: molecular size markers, 1: solubilized and refolded GST–Cap-Pa28; 2: Purified GST–Cap-Pa28; 3: Cleaved Cap-Pa28, after thrombin treatment. b Induction of the hypersensitive response by GST–Cap-Pa28 in tobacco (Nicotiana glutinosa) leaves. CAP, purified Cap-Pa28 from Phytophthora capsici Pa28; GST–CAP, GST–Cap-Pa28. The hypersensitive response was detected 18 h after the treatment of 10–50 nmol of protein