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Purification and autophosphorylation assay of GST–NgRLK1522–789. a SDS–PAGE analysis of GST–NgRLK1522–789. M, molecular size markers; 1, Non-induced; 2, IPTG-induced; 3, Purified GST–NgRLK1522–789. GST–NgRLK1522–789 was detected by CBB staining. b autophosphorylation activity of GST–NgRLK1522–789. Autoradiogram (right) and stained gel (left) are shown
