Table 1.
Bacterial strains and plasmids
| Strains/plasmids | Characteristicsa | Sources/references |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | F− Φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 deoR recA1 hsdR17(rK − mK +) phoA supE44 λ − thi-1gyrA96 relA1 | Gibco-BRL |
| BL21 | F− ompT hsdS B(rB − mB +) gal dcm | Takara |
| BL21(DE3) | F− ompT hsdS B(rB − mB +) gal dcm λ(DE3) | Novagen |
| Top10 | F− mcrA ∆(mrr-hsdRMS-mcrBC) Φ80lacZ∆M15 ∆lacΧ74 recA1 araD139 ∆(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| Saccharomyces cerevisiae | ||
| AH109 | MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4∆, gal80∆, LYS2::GAL1 UAS -GAL1 TATA -HIS3, GAL2 UAS -GAL2 TATA -ADE2, URA3::MEL1 UAS -MEL1 TATA -lacZ | Clontech |
| Phytophthora capsici | ||
| Pa28 | Wild type (a Korean isolate) | NIAST, RDAb |
| Plasmids | ||
| pGEX-2T | tac promoter-based expression vector; Ampr | GE Healthcare Bioscience |
| pGEX4T-1 | tac promoter-based expression vector; Ampr | GE Healthcare Bioscience |
| pCR4.1-TOPO | Cloning and sequencing vector; Ampr | Invitrogen |
| pGEM-T easy | Cloning and sequencing vector; Ampr | Promega |
| pGADT7-Rec | AD/library, LEU2, HA epitope; Ampr | Clontech |
| pGADT7 | AD/library, LEU2, HA epitope; Ampr | Clontech |
| pGBKT7 | DNA/bait, TRP1, c-Myc epitope; Kmr | Clontech |
| pGADT7-T | AD/T-antigen, control vector (yeast-two hybrid); Ampr | Clontech |
| PGBKT7-53 | DNA-BD/p58, control vector (yeast-two hybrid); Kmr | Clontech |
| pCold™II | cspA promoter-based cold shock expression vector; Ampr | Takara |
| pGEMCAP1 | Cap-Pa28 in pGEM-T Easy; Ampr | This study |
| pGEXCAP1 | Cap-Pa28 in pGEX-2T; Ampr | This study |
| pBDCAP1 | Cap-Pa28 in pGBKT7; Kmr | This study |
| pADNG-238 | 2,749 bp cDNA fragment including entire NgRLK1 from N. glutinosa cloned into pGADT7-Rec; Ampr | This study |
| pAD-RLK1 | NgRLK1 in pGADT7; Ampr | This study |
| pAD-RLK1out | NgRLK1 23−438 in pGADT7; Ampr | This study |
| PAD-RLK11n | NgRLK1 522−789 in pGADT7; Ampr | This study |
| pGX4-RLK1in | NgRLK1 522−789 in pGEX4T-1; Ampr | This study |
| pCD-RLK1out | NgRLK1 23−438 in pCold™II; Ampr | This study |
| pCD-RLK1in | NgRLK1 522−789 in pCold™II; Ampr | This study |
aAmpr, ampicillin resistance; Kmr, kanamycin resistance
bNational Institute of Agricultural Science and Technology, Rural Development Administration