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. 2010 Jan;176(1):51–63. doi: 10.2353/ajpath.2010.080843

Figure 1.

Figure 1

The rNephrin transgenic mouse. A: The JRC-rNephrin transgene construct: In a binary reverse doxycycline-regulated expression vector an NPHS2 (podocin) promoter drives the expression of the recombinant inducible transcription factor reverse tetracycline transactivator (M2). In the presence of doxycycline, reverse tetracycline transactivator binds to the tetO7 element linked to a minimal CMV promoter that drives the expression of rat nephrin cDNA. B: Rat- and mouse-specific nephrin mRNA expressions in JRC-rNephrin (rNeph) mouse kidneys isolated from nine founder-lines (93A – 66A) analyzed by RT-PCR. C: RT-PCR of mRNA from the kidney cortex of the selected JRC-rNephrin founder-line (86A): 1) Wild-type mouse without doxycycline, 2) rNeph mouse without doxycycline, and 3) rNeph mouse with doxycycline. Total RNA was isolated from kidneys collected from adult mice at the age of 10 to 12 weeks after 2 weeks of doxycycline (0.2 mg/ml) administration. PCR was performed using primers presented in Table 2. Similar results were obtained with three independent experiments with three mice in each genotype.