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Expressions of rat nephrin, mouse nephrin, podocin, and WT1 mRNAs analyzed by RT-PCR. Total RNA was isolated from the kidneys of E11 and E14 wild-type (WT) and rNeph embryos collected from pregnant rNeph transgenic mouse line females with 2.0 mg/ml doxycycline in the drinking water. The primers used are listed in Table 2. Similar results were obtained with three independent experiments with three mice in each genotype.
