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. 2010 Jan;176(1):51–63. doi: 10.2353/ajpath.2010.080843

Figure 3.

Figure 3

Expression of transgenic rat nephrin in podocytes rescues conventional nephrin KO mice from perinatal lethality with normal kidney and podocyte morphology at the age of 1 week. A: Mouse- and rat-specific nephrin mRNA expressions in rNeph/mNeph mice kidney cortex at the age of 1 week, as analyzed by RT-PCR. Genotypes are marked 1) rNeph/mNeph+/+, (2) rNeph/mNeph−/− and (3) rNeph/mNeph+/−. B and C: Normal nephrin protein expression and localization in rNeph/mNeph+/+ and rNeph/mNeph−/− mice at the age of 1 week analyzed by immunofluorescent microscopy of frozen kidney sections (magnification ×100). D and E: Kidney histology in rNeph/mNeph+/+ and rNeph/mNeph−/− mice at the age of 1 week analyzed by H&E staining of paraffin-embedded kidney sections (×200 magnification). F and G: Electron microscopy shows relatively normal podocyte fine structures and well-preserved slit diaphragm structures in kidneys of rNeph/mNeph+/+ and rNeph/mNeph−/− mice. Arrows indicate existing slit diaphragms (SDs). Glomerular basement membrane (GBM); urinary space (US). Scale bars = 200 nm.