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. 2010 Jan;176(1):51–63. doi: 10.2353/ajpath.2010.080843

Figure 6.

Figure 6

Changes expression of podocin, Nck2, and WT1 mRNAs in 6-week-old rescued rNeph/mNeph−/− mice. RT-PCR was performed with total RNA isolated from kidney cortex at the age of 6 weeks. Genotypes are marked: 1) rNeph/mNeph+/+, 2) rNeph/mNeph−/−, and 3) rNeph/mNeph+/−. A and B: Podocin and Nck 2 expression was decreased in rescued rNeph/mNeph−/− mice kidneys. C: WT1 expression was analyzed using a primer set designed to amplify different splicing forms with or without exon 5. The results showed an increase in the exon 5 containing mRNA variant in rescued rNeph/mNeph−/− mice, as compared with the control genotypes. D: β-actin expression was similar in all genotypes. Primers and size of the products are listed in Table 2. Similar results were obtained in three independent experiments with three mice of each genotype.