Figure 7.

Changed podocin and Fyn protein expression in 6-week-old rescued rNeph/mNeph^−/− mice. Immunofluorescent double-staining of frozen kidney sections from rNeph/mNeph^+/+ (left panel) and rNeph/mNeph^−/− (right panel) mice with podocin-, Fyn-, and synaptopodin-specific antibodies showed that podocin protein is markedly decreased in the rescued mice, as compared with control mouse kidneys (two upper rows). Instead, the Fyn protein levels in the area close to the podocyte foot processes and slit diaphragm were increased in the rescued mice (two lowest rows). Magnification: ×200.