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. 2010 Jan;176(1):51–63. doi: 10.2353/ajpath.2010.080843

Figure 9.

Figure 9

A: Immunofluorescent staining with WT1 and synaptopodin specific antibodies on frozen kidney sections from 6-week-old rNeph/mNeph+/+, and rescued rNeph/mNeph−/− mice. Surprisingly, WT1 expression was absent in the nucleus of 6-week-old rescued rNeph/mNeph−/− mice podocytes when still present in age-matched control kidneys. Magnification: ×200. B: Immunoblotting from kidney cortex lysate with nephrin antibody showed an increased level of the phosphorylated nephrin form (∼180 kDa) rNeph/mNeph−/− mice, as compared with the other genotypes (Figure 9B). C: Quantitative immunoblotting experiments verified RT-PCR and immunofluorescent microscopic findings that expression level of podocin and Nck2 has been decreased (***P < 0.001) and Nck1 unchanged in rescued mice as compared with control rNeph/mNeph+/+ littermates.