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. 2010 Jan;176(1):108–121. doi: 10.2353/ajpath.2010.090256

Figure 3.

Figure 3

SiRNA knockdown of FGFR1OP2/wit3.0 attenuated collagen gel contraction of oral wound fibroblasts. A: Floating collagen gel contraction assay showed the increased gel contraction by oral wound fibroblasts (W-OFs) as compared with naïve oral fibroblasts (OFs) and skin fibroblasts (SFs) at 12 hours and 24 hours (*P < 0.05). B: Efficacy of five siRNA constructs was evaluated by real time RT-PCR (*P < 0.05 against negative control). The treatment by siRNAs number 4 and number 5 did not affect the expression of contractile and procontractile molecules: α-SMA, radixin, ezrin, and moesin. C: FACS analysis demonstrated the establishment of the optimal transfection protocol for primary oral wound fibroblasts achieving >80% transfection rates (black line) compared with untransfected control (gray line). D: Floating collagen gel contraction assay of oral wound fibroblasts showed that FGFR1OP2/wit3.0 siRNA number 5 significantly attenuated gel contraction (*P < 0.05).