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. 2010 Jan;176(1):108–121. doi: 10.2353/ajpath.2010.090256

Figure 5.

Figure 5

FGFR1OP2/wit3.0β overexpression increased collagen gel contraction by both oral and skin fibroblasts. A: Nonsynonymous SNPs in human FGFR1OP2/wit3.0β sequence. B: A schematic structure of lentiviral vector carrying humanized FGFR1OP2/wit3.0β (wild-type, SNP1, SNP2, or SNP1/2) and internal ribosome entry site-green fluorescent protein. C: FACS analysis of the transduction rate of the lentiviral vector for primary oral fibroblasts assessed by green fluorescent protein. D: Floating collagen gel contraction assay revealed that both oral and skin fibroblasts transduced by FGFR1OP2/wit3.0β lentiviral vectors (dotted lines) increased gel contraction rates. E: SNP1, SNP2, and SNP1/2 of FGFR1OP2/wit3.0β increased collagen gel contraction rates. In both oral and skin fibroblasts, SNP1 FGFR1OP2/wit3.0β showed the largest gel contraction rate. F: The treatment of TGF-β1 did not modulate the expression of FGFR1OP2/wit3.0 in oral and skin fibroblasts, whereas it increased the α-SMA expression. *P < 0.05, **P < 0.01.