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. 2010 Jan;176(1):146–157. doi: 10.2353/ajpath.2010.080639

Figure 7.

Figure 7

siRNA knockdown results in decreased Caco-2 cell proliferation and reduced bFGF-dependent MAPK signaling. Caco-2 cells were subjected to siRNA mediated knockdown of syndecan-1 mRNA expression as described in the Methods section. A: Quantitative real-time PCR analysis confirms successful knockdown of Sdc1 expression by >50% (n = 3, P < 0.01). B: Cell proliferation is significantly reduced in Sdc1-silenced Caco-2 cells (n = 3, P < 0.001). C: Western blot analysis of bFGF-mediated p44/42 MAPK activation in Sdc1-silenced Caco-2 cells. Caco-2 cells were transfected with a control siRNA or a Sdc1 siRNA construct and serum-starved for 24 hours after transfection. Cells were treated ±20 nmol/L bFGF for 0, 10, or 20 minutes, lysed, and analyzed by Western blotting for phospho-p44/42 MAPK and total p44/42 MAPK.