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. 2010 Jan;176(1):187–197. doi: 10.2353/ajpath.2010.090322

Figure 2.

Figure 2

mRNA expressions of PPT-A, IL-4, IL-6, TNF-α, and IFN-γ in the pinnae taken from the double-stressed CH mice relative to the unstressed mice. Their expressions were analyzed by real-time PCR. Relative expression of real-time PCR products was determined by the Ct method to compare target gene and GAPDH mRNA expressions. All values represent the mean ± SEM, which indicate mRNA expressions in stressed CH mice relative to those in unstressed CH mice 48 hours after elicitation. Stressed CH mice showed significant increases in all parameters relative to the unstressed CH mice (*P < 0.05). These results were obtained from 8 to 10 mice.