Figure 2.

H-PGDS expression in pDCs and mDCs. A: Skin tissues from patients with atopic dermatitis were stained with BDCA-2-FITC Ab, a specific marker for pDCs, or BDCA-1-FITC Ab, a specific marker for mDCs. H-PGDS was co-localized with BDCA-2⁺ and BDCA-1⁺ pDCs and mDCs, respectively (arrowheads in upper and lower rows). pDCs were confirmed by positive staining for CD123 (arrowheads in the middle row). B: Flow cytometric analysis for intracellular H-PGDS in pDC and mDC with whole blood staining. pDCs and mDCs in whole blood were identified as CD123⁺/BDCA-2⁺ cells and CD19⁻/BDCA-1⁺ cells, respectively. Intracellular H-PGDS in these cells was detected by staining with polyclonal anti-human H-PGDS Ab.