Figure 4.

Morphological analysis of peritoneal lavage cells. Cells from the peritoneal cavity at day 2 after implantation stained for Gr1 (A) or F4/80 (D). Morphological analysis (Diff-Quik) of FACS-sorted EGFP^loGr1^lo (green) and EGFP^hiGr1^hi (red) subsets shows neutrophils (B) and polymorphonuclear leukocytes and mononuclear cells with ring-shaped nuclei (arrow) (C), respectively. FACS-sorted EGFP^loF4/80^lo cells were monocytes and neutrophils (E), whereas the EGFP^hiF4/80^hi subset comprised macrophages (F). Scale bars = 10 μm.