Table 2.

Methylation Profile of Eight Genes in Normal PBMC, HTLV- I Carrier, and Various Types of ATLL

Normal PBMC (n = 16)
Age	Sex	SHP1	p15	p16	p73	hMLH1	MGMT	DAPK	HCAD	CIMP
49	M
46	M
31	M
29	F
26	F		+
29	F		+						+
27	F
54	M								+
43	M								+
30	F
29	F								+
52	M		+
43	M
56	F
57	F								+
40	M								+
% methylation		0%	19%	0%	0%	0%	0%	0%	38%	0%
		(0/16)	(3/16)	(0/16)	(0/16)	(0/16)	(0/16)	(0/16)	(6/16)	(0/16)
HTLV-1 carrier (n = 10)
50	F
52	F						+
38	M				+
49	M	+
57	M						+
42	M								+
72	F	+
34	F	+	+		+					+
36	M		+					+	+	+
52	F				+	+		+	+	+
% methylation		30%	20%	0%	30%	10%	20%	20%	30%	30%
		(3/10)	(2/10)	(0/10)	(3/10)	(1/10)	(2/10)	(2/10)	(3/10)	(3/10)
Smoldering type (n = 15)
66	M								+
56	F								+
62	F								+
53	M								+
48	F	+							+
43	F			+					+
63	M							+	+
54	M							+	+
48	F							+	+
51	F		+						+
58	M	+	+						+	+
55	F				+			+	+	+
79	M		+					+	+	+
49	F						+	+	+	+
71	M	+	+	+				+	+	+
% methylation		20%	27%	13%	7%	0%	7%	47%	100%	33%
		(3/15)	(4/15)	(2/15)	(1/15)	(0/15)	(1/15)	(7/15)	(15/15)	(5/15)
Chronic type (n = 5)
64	M								+
68	M	+						+	+	+
34	F				+		+	+	+	+
47	M	+	+				+		+	+
59	F	+	+					+	+	+
% methylation		60%	40%	0%	40%	0%	40%	60%	100%	80%
		(3/5)	(2/5)	(0/5)	(1/5)	(0/5)	(2/5)	(3/5)	(5/5)	(4/5)
Acute type (n = 15)
60	F
64	M					+			+
60	M	+
58	M							+	+
73	M	+							+
45	F	+							+
65	F	+							+
47	F	+				+			+	+
67	F	+						+	+	+
67	F	+						+	+	+
53	F	+						+	+	+
53	F	+						+	+	+
55	F	+	+					+	+	+
72	M	+	+					+	+	+
73	M	+	+			+		+	+	+
% methylation		80%	20%	0%	0%	20%	0%	53%	87%	53%
		(12/15)	(3/15)	(0/15)	(0/15)	(3/15)	(0/15)	(8/15)	(13/15)	(8/15)
Lymphoma type (n = 20)
47	M								+
70	M						+	+
47	M	+	+						+	+
74	M	+					+	+		+
70	M	+		+					+	+
59	F	+						+	+	+
41	M	+						+	+	+
64	F	+						+	+	+
77	M	+						+	+	+
57	M	+		+		+			+	+
36	F	+		+				+	+	+
74	M	+					+	+	+	+
47	M	+					+	+	+	+
67	M	+		+				+	+	+
69	M	+		+			+	+	+	+
82	F	+		+	+			+	+	+
48	F	+	+				+	+	+	+
67	F	+	+		+		+	+	+	+
58	M	+		+	+		+	+	+	+
75	M	+	+	+			+	+	+	+
% methylation		90%	20%	40%	15%	5%	45%	80%	90%	90%
		(18/20)	(4/20)	(8/20)	(3/20)	(1/20)	(9/20)	(16/20)	(18/20)	(18/20)
Total % methylation		SHP1	p15	p16	p73	hMLH1	MGMT	DAPK	HCAD	CIMP
		60%	23%	15%	12%	8%	22%	55%	63%	58%
		(39/65)	(15/65)	(10/65)	(8/65)	(5/65)	(14/65)	(36/65)	(41/65)	(38/65)

The methylation profile, showing the + represents methylated specimens, while the blank columns represent unmethylated specimens. M, male; F, female.