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. 2010 Jan;176(1):446–460. doi: 10.2353/ajpath.2010.090383

Figure 2.

Figure 2

Delayed ductal outgrowth in MMTV/CrkII transgenic mice compared with normal FVB littermates. A: Delayed ductal outgrowth in transgenic mice was detected at 5 weeks (data not shown), 10 weeks (upper right panel), and 12 weeks (lower right panel) relative to FVB mice at the same age (upper and lower left panels). Images taken at ×0.6. Arrows represent the terminal end bud structures. B: The percentage of fat pad filled by epithelial ducts was calculated using Scion Image for six FVB mice and six CrkII mice at 10 weeks of age and the average percentage was determined ± SE (ii), P < 0.05. The distance from the lymph node to the terminal end bud over the distance of the lymph node to the end of the fat pad was determined and expressed as a percentage. C: The average number of terminal end bud structures among FVB (n = 6) and MMTV/CrkII mice (n = 6) and the percentage of increased diameter size of these structures within CrkII mice relative to FVB control mice ± SE, P < 0.05. D: Paraffin-embedded sections of 10-week-old mammary glands from six FVB and six MMTV/CrkII mice were stained for Ki-67 and further comparison of FVB terminal end buds to those from MMTV/CrkII transgenic mice were examined using the ApopTag Plus Peroxidase In Situ Detection system. Staining patterns of CK8 and CK14 were also visualized in FVB versus CrkII transgenic mice. All images taken at ×20; scale bar = 50 μm. Arrows represent positive staining.