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. 2010 Jan;176(1):446–460. doi: 10.2353/ajpath.2010.090383

Figure 7.

Figure 7

Enhanced CTGF and Netrin-4 protein levels are found in mammary tissue derived from MMTV/CrkII transgenic mice. A: Western blot analysis illustrating enhanced CTGF and Netrin-4 levels in MMTV/CrkII transgenic mice relative to wild-type FVB mice at 20 weeks of age. B: Densitometry of CTGF and netrin-4 in relation to actin levels. The ratio of CTGF to actin per mouse was calculated and the average ratios from the wild-type mice and the MMTV/CrkII transgenic mice were compared, ± SE. Netrin-4 levels were calculated in the same manner. C: Immunohistochemistry on paraffin sections from mice at 10 weeks of age reveals positive netrin-4 staining surrounding the terminal end buds of transgenic mice (see black arrows) with very little positive netrin-4 staining in FVB littermates. Laminin is present in both wild-type and MMTV/CrkII transgenic mice (see black arrows). All images taken at ×20. Scale bar = 50 μm.