Figure 4.

Two-chamber co-culture with retinal cells under hypoxic conditions enhances EPC cell migration. In a transwell co-culture system under hypoxic conditions with an 8 μm hole-size (purple) filter, many cells (yellow) stayed in the upper chamber (A) and few cells passed through the filter to the lower chamber (C) when co-cultured for 6 hours with HMVECs. In contrast, after co-culture with retinal cells, many more EPCs passed through the holes into the lower chamber (D) and fewer cells stay in the upper chamber (B).