Skip to main content
. 2009 Oct 13;59(1):119–127. doi: 10.2337/db09-0512

FIG. 7.

FIG. 7.

Overexpression of PKCδWT and PKCδKN changes FOXO1 cellular distribution, phosphorylation, and protein concentration in insulin-secreting cells. INS-1E cells and INS-1E cells overexpressing PKCδWT or PKCδKN were cultured under control conditions and in the presence of 0.6 mmol/l palmitate for 1 h and 1 day. A: Representative Western blot for FOXO1 detection in cytosolic fractions. B and C: Representative Western blot for FOXO1 detection in nuclear fractions with means ± SEM of n = 4 independent experiments. D–F: Representative Western blot for FOXO1, P-Ser256-FOXO1, and tubulin (as loading control) of cell homogenates with means ± SEM of n = 4 independent experiments of relative amounts of FOXO1 and phosphorylation of FOXO1 (P-FOXO1/FOXO1) by setting the respective bands of control INS-1 cells to 100%. *Significant difference to INS-1E under control condition; §significant difference to the respective condition of INS-1E; #significant difference to PKCδKN at the same condition.