FIG. 2.

Accumulation of DNA damage and elevated p53 and p21 in Lig4^−/−p53^p/p mice. A: Pancreatic sections were stained for insulin by immunofluorescence (red), γH2AX foci (green), and DAPI (blue) in 2- and 4-month-old Lig4^−/−p53^p/p and wild-type mice. γH2AX foci visualized in Lig4^−/−p53^p/p are more intense in 4- than in 2-month-old mutant mice. No γH2AX foci were visible in wild-type mice. Double immunohistochemical staining of pancreatic sections from 2- or 3-month-old Lig4^−/−p53^p/p and wild-type mice for (B) p21 and (C) p53 (black) in combination with insulin (red-brown). Magnification ×40. Mon, month; WT, wild type. (A high-quality color digital representation of this figure is available in the online issue.)