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. 2009 Oct 15;59(1):17–25. doi: 10.2337/db09-0929

FIG. 4.

FIG. 4.

Triglyceride lipolysis and fatty acid β-oxidation genes increase in WAT of leucine-deprived mice. Expression of triglyceride lipolysis in WAT of mice fed a control, leucine-deficient, or pair-fed diet for 7 days is shown. Data are means ± SE of at least two independent real-time PCR experiments (D and E) or Western blot (A and F) with mice of each diet for each experiment (control diet, n = 6; (−) leu diet, n = 6; pair-fed diet, n = 6). Statistical significance was determined by one-way ANOVA followed by the Student-Newman-Keuls test for the effect of either (−) leu or pair-fed diet versus control diet (*P < 0.01) or (−) leu diet versus pair-fed diet (#P < 0.01). A: p-HSL, HSL, and p-PKA substrate proteins. B: Glycerol release assay. C: cAMP content. D: Adrb3 mRNA. E: Pparα, Cpt-1, and Aco1 mRNA. F: PPARα protein.