Skip to main content
. 2009 Oct 15;59(1):249–255. doi: 10.2337/db09-0801

FIG. 4.

FIG. 4.

Hyperglycemia-induced methylglyoxal increases binding of NFκB to the RAGE promoter and binding of AP-1 to the S100A8, S10012, and HMGB1 promoters. A: Conditionally immortalized HAECs were treated with low glucose (LG), high glucose (HG), or HG after transfection with either scrambled oligonucleotides (LG and HG) or siRNA for p65 (HG/siP65) for 5 days, and the RAGE mRNA level was determined by qPCR (n = 3). B: Primary HAECs were treated with LG, HG, or HG after infections with either nontarget control or GLO1 adenovirus (HG/GLO1) for 5 days. Chromatin immunoprecipitation was performed using the p65 antibody, and the RAGE promoter was amplified by qPCR (n = 4). C: Conditionally immortalized HAECs were treated with LG, HG, or HG after transfection with either scrambled oligonucleotides or siRNA for AP-1 (c-Jun) (HG/siAP-1) for 5 days, and mRNA levels of S100A8, S10012, and HMGB1 were determined by qPCR (n = 3). D: Primary HAECs were treated with LG, HG, or HG after infections with either nontarget control or GLO1 adenovirus (HG/GLO1) for 5 days. Chromatin immunoprecipitation was performed using c-Jun antibody, and the S100A8, 100A12, and HMGB1 promoters were amplified by qPCR (n = 4). Values are shown as means + SD. *P < 0.05 vs. LG group. (A high-quality color digital representation of this figure is available in the online issue.)