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. Author manuscript; available in PMC: 2009 Dec 30.
Published in final edited form as: J Biochem. 2008 Mar 13;143(6):821–832. doi: 10.1093/jb/mvn035

Fig 2. Oligomeric states of the mutated VE-EC1-4 fragments.

Fig 2

The oligomeric state of the fragments was studied by analytical gel filtration chromatography as previously described (Bibert et al. 2002). All the chromatographic runs were performed at 4°C.

Panel A : Impact of mutations on the molecular exclusion profiles of the VE-EC1-4 mutants.

FPLC molecular exclusion profiles for VE-EC1-4 mutants were obtained at initial loading concentrations of 17 μM (left) or 56 μM (right).

Panel B : Kinetic study of the mutant association

The different mutants were concentrated at 17 μM and immediately analyzed by gel filtration chromatography (left). Then, they were stored at room temperature during 24 hours before being analyzed again by gel filtration chromatography (right).