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. Author manuscript; available in PMC: 2009 Dec 30.
Published in final edited form as: J Biochem. 2008 Mar 13;143(6):821–832. doi: 10.1093/jb/mvn035

Fig 3. Determination of the oligomeric state of VE-EC1-4M3 by cross-linking experiments.

Fig 3

VE-EC1-4M3 (17 μM) was cross-linked using EDC at various concentrations: 0 mM (lane 2), 40 mM (lane 3), 80 mM (lane 4), 160 mM (lane 5), 320 mM (lane 6) and 640 mM (lane 7). Lane 1 : molecular weight markers. Cross-linked products were analyzed by SDS electrophoresis using a 5-20 % gradient gel. The oligomeric states of each cross-linked product are indicated on the right (X1: monomer; X2: dimer).