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. Author manuscript; available in PMC: 2009 Dec 30.
Published in final edited form as: Circ Res. 2005 Jan 20;96(3):384–391. doi: 10.1161/01.RES.0000156652.99586.9f

FIGURE 2. Effect of peroxovanadate treatment on tyrosine phosphorylation pattern in adult tissues.

FIGURE 2

Panel A: The pattern of tyrosine phosphorylated proteins were analyzed in mice treated with peroxovanadate or vehicle alone. After 5 min of treatment, the animals were sacrificed and tissue proteins were extracted. Samples containing 10 μg of total proteins were analyzed by Western blot with the anti-phosphotyrosine antibody. This analysis was performed for each treated mouse to check that the treatment was successful. Panel B: Evaluation of VE-cadherin phosphorylation in peroxovanadate-treated adult tissues. VE-cadherin immunoprecipitates were Western blotted with the anti VE-cadherin antibody and the anti-phosphotyrosine antibody, sequentially. Lung extracts were analyzed separately because of the much higher VE-cadherin content of this organ. The anti-phosphotyrosine antibody only labeled the full-length VE-cadherin (arrow) and not the truncated form below, devoid of the cytoplasmic domain.