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. Author manuscript; available in PMC: 2009 Dec 30.
Published in final edited form as: Circ Res. 2005 Jan 20;96(3):384–391. doi: 10.1161/01.RES.0000156652.99586.9f

FIGURE 3. VE-cadherin tyrosine phosphorylation in hormone-stimulated ovary and uterus.

FIGURE 3

Panel A: Female mice were stimulated with PMSG/hCG and treated with peroxovanadate (see “Materials and Methods”). Tyrosine phosphorylated proteins (10 μg) were analyzed by Western blot with the anti-phosphotyrosine antibody. Panel B: VE-cadherin was immunoprecipitated from 1 mg of proteins from ovary, uterus and lung extracts. Immunoprecipitates were immunoblotted with the anti-phosphotyrosine antibody, stripped and re-probed with anti-VE-cadherin antibody. Panel C: Western blots were semiquantified by densitometry for calculation of the ratio of the phosphorylated versus total VE-cadherin in the immunoprecipitates. Data are presented as fold induction by hormone treatment. This experiment is representative of two additional experiments.