FIGURE 4. Flk1/VE-cadherin protein association in mouse ovary and uterus stimulated by hormones.
Panel A: Mice were treated with PMSG/hCG or vehicle alone, and peroxovanadate. Flk1/VE-cadherin association was revealed by immunoprecipitation of VE-cadherin from 1 mg of tissue protein lysates, immunoblotting with the anti-Flk1 and the anti-VE-cadherin antibodies. This experiment is representative of two additional experiments. Panel B: Flk1-VE-cadherin association was confirmed by immunoprecipitation of uterus protein extracts (1 mg) with the anti-Flk1 antibody and immunoblotting with the anti-Flk1 and the anti-VE-cadherin antibodies. The lower band (50 kDa) is the Ig-heavy chain.