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. Author manuscript; available in PMC: 2009 Dec 30.
Published in final edited form as: Circ Res. 2005 Jan 20;96(3):384–391. doi: 10.1161/01.RES.0000156652.99586.9f

FIGURE 5. Src/VE-cadherin association and Src phosphorylation state in mouse ovary and uterus.

FIGURE 5

Panel A: Mice were treated by PMSG/hCG or vehicle alone, and peroxovanadate. Src/VE-cadherin association was analyzed in VE-cadherin immunoprecipitates from protein lysates (0.5 mg for lung and 1 mg for ovary and uterus) and immunoblotting with the anti-Src antibody. The membrane was stripped and reprobed with the anti-phosphotyrosine antibody. The lower band (50 kDa) is the Ig-heavy chain. Panel B: To confirm Src/VE-cadherin association, uterus extracts (1 mg) were immunoprecipitated with anti-Src antibody. Proteins were then Western blotted with the anti-VE-cadherin and the anti-Src antibodies. This experiment is representative of three additional experiments.