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. Author manuscript; available in PMC: 2009 Dec 30.
Published in final edited form as: Circ Res. 2005 Jan 20;96(3):384–391. doi: 10.1161/01.RES.0000156652.99586.9f

Figure 6. Src inhibitors impaired VEGF-induced VE-cadherin phosphorylation in endothelial cells but not VE-cadherin-Src association.

Figure 6

Panel A: Confluent cultures of HUVEC were serum starved (1% serum) for 6 h and incubated with 50 ng/ml VEGF for 0 to 30 min. Proteins were immunoprecipitated with anti-VE-cadherin and Western blotted with anti-P-tyr and anti-VE-cadherin antibodies. Panel B: Src inhibitors prevented VEGF-induced VE-cadherin phosphorylation. VE-cadherin was immunoprecipitated from VEGF-stimulated HUVEC treated with increasing concentrations of SU6656 or PP2, as indicated. The presence of P-tyr- VE-cadherin was detected as above. Panel C: Src inhibitor did not disrupt VE-cadherin-Src association. VE-cadherin was immunoprecipitated from HUVEC treated with VEGF and increasing concentrations of SU6656. VE-cadherin and Src were revealed by Western blotting. Panel D: Immunolocalization of Src and VE-cadherin in confluent HUVEC. Double immunofluorescence staining (Src, green; VE-cadherin, red) showed protein colocalization at cell-cell junctions (yellow staining in merged images).