Figure 2.

Primary satellite cells express a larger number of integrin mRNAs than cell lines: C2C12 cells express some integrin proteins differently compared to primary cells and MM14 cells. (A): Expression of integrin mRNAs in C2C12, MM14, and primary satellite cell-derived myoblasts; identical samples were processed simultaneously. Unlike immortalized cell lines, primary cells are positive for most or all known integrin chains (some samples of primary cells were positive for expression of integrins αE and αL, but not all were, including the one shown here). Intron-spanning primer sequences may be found in Supporting Table 1. (B): Expression of integrin protein in primary satellite cells (PSC), MM14, and C2C12 cells: all blots were stripped and reprobed with IP90 (calnexin) as a loading control; shown is anti-αV after stripping and reprobing.