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. 2009 Oct;27(10):2527–2538. doi: 10.1002/stem.178

Figure 4.

Figure 4

Fiber-associated satellite cells exhibit multiple novel “behaviors.” Figures are reference stills taken from the associated movies; magnified regions are shown below full-scale images. (A): Two movies showing recently-divided sister cells remaining closely associated for extended periods while moving along the fiber. Movie 4a1: Inclusive time 24 to 48 hours after activation, stills taken at 28:30; 28:40; 30:10; 32:10; 33:20; 45:40 hours. Movie 4a2: inclusive time 24 to 51 hours after activation, stills taken at 30:00; 30:30; 32:20; 34:30; 35:20; 48:50 hours. (B): Beginning at 12 hours (prior to any cell division), two presumably unrelated satellite cells migrate toward each other, associate, and remain closely associated for an extended period. Movie 4b: inclusive time 12 to 36 hours after activation, stills taken at 21:40; 25:20; 25:50; 26:30; 29:20; 36:00 hours. (C): Multiple satellite cells remain very closely associated for an extended time, interacting extensively but not fusing. Movie 4c: Inclusive time 48 to 72 hours after activation, stills taken at 48:00; 54:20; 59:20; 61:30; 69:10; 72:00 hours. Note that while some apparent “planar” cell divisions can be (as in still 2) other cell associations that appear to be planar cell divisions are almost certainly not (as in still 3). (D): A cell repeatedly extends protrusions into the collagen matrix, then moves off the fiber; another cell moves out along the first cell, divides, and the daughter cells continue in opposite directions. Movie 4d: inclusive time 48 to 72 hours after activation; stills taken at 52:20; 63:20; 65:20; 66:40; 67:10; 68:00 hours. (E): A cell in the collagen matrix moves parallel to a fiber, turns, and migrates onto the fiber. Movie 4e: Inclusive time 62 to 70 hours after activation, stills taken at 64:00; 65:10; 66:50; 67:20; 68:00; 68:50 hours. Note: area of magnified image is moved from frame to frame. (F): Relative frequencies of described activities in FGF-2-treated cultures from 24 to 48 hours after harvest. See Supporting Table 2, supporting movie Information, and additional Quicktime movies for more detailed information.