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. Author manuscript; available in PMC: 2009 Dec 27.
Published in final edited form as: Pharm Res. 2009 Mar 4;26(6):1432–1445. doi: 10.1007/s11095-009-9853-y

Fig. 1.

Fig. 1

Key constructs involved in targeting lentiviral vectors to T-cells. FUGW: a lentiviral backbone construct encoding green fluorescence protein (GFP) as a reporter gene controlled by a human ubiquitin-C promoter (Ubi). WRE: woodchuck responsive element; CMV enhancer: the enhancer element derived from human cytomegalovirus. OKT3: a construct encoding the membrane-bound human/mouse chimeric anti-CD3 antibody, as well as two promoters to drive the expression of the light and heavy chains of the antibody. CMV: human cytomegalovirus immediate-early gene promoter; EF1α: human elongation factor 1 α promoter; OKT3 Vκ: the variable domain of the kappa chain of the mouse OKT3 antibody; Human Cκ: the constant domain of the kappa chain of human immunoglobulin; OKT3 Vγ: the variable domain of the gamma chain of the mouse OKT3 antibody; Human Cγ: the constant domain of the gamma-1 chain of human immunoglobulin; TM: the transmembrane domain of the gamma-1 chain of human immunoglobulin; pA: polyadenylation signal. pIgαβ: a construct encoding the accessory proteins for human immunoglobulin, Igα and Igβ. Fusogen: a mutant fusogenic molecule derived from Sindbis virus glycoprotein. Indicated mutations were introduced to the fusion loop to generate the new variants of fusogen, SV2, SV3, and SV4.