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. Author manuscript; available in PMC: 2009 Dec 27.
Published in final edited form as: Pharm Res. 2009 Mar 4;26(6):1432–1445. doi: 10.1007/s11095-009-9853-y

Fig. 6.

Fig. 6

The effects of antibody competition and endosomal neutralization on lentiviral transduction in vitro. (a) Gradient concentrations of NH4Cl (dissolved in PBS) were pre-mixed with lentiviral supernatants (FUGW/OKT3+SV3) and added to Jurkat.CD3 cells for transduction. After replacement of the supernatant with fresh culture medium, the cells were incubated for another three days before FACS analysis of GFP expression. All of the data was normalized by the transduction efficiency compared to transduction without NH4Cl treatment. (b) Jurkat.CD3 cells were pre-incubated with gradient concentrations of soluble OKT3 antibody (OKT3) for 30 min, after which fresh unconcentrated FUGW/OKT3+SV3 supernatant (1 ml) was added for transduction. After replacement of the supernatant with fresh culture medium, the cells were incubated for additional three days before FACS analysis. The isotype-matched antibody was included in the experiment as a control.