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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cytometry A. 2009 Oct;75(10):866–873. doi: 10.1002/cyto.a.20773

Figure 4.

Figure 4

His-tag purification: N-terminal PSGL-1 (HT) peptide from CHO/HL-60 cells obtained following thrombin treatment was purified using MagneHis beads. 500mM imidazole was used to elute peptides from these nickel beads. The presence of peptide in this eluate was determined using TB5-beads to capture PSGL-1 (HT) and anti-tetra-His mAb for detection. Data are presented as mean + SEM (N=3). Fluorescence signal from eluted peptide is higher than original sample due to sample concentration that occurs during purification.