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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Cytometry A. 2009 Dec;75(12):1031–1039. doi: 10.1002/cyto.a.20790

Figure 1. Laser light contamination of fluorescein and PE detectors.

Figure 1

532, 550 or 561 nm lasers were mounted and aligned on a BD LSR II™, and MESF FITC low microspheres (left two columns) and MESF PE low microspheres (right two columns) were subsequently analyzed in the 488 nm aligned fluorescein and PE detectors respectively (spatially separated from the 532, 550 or 561 nm optical path). A 510/20 nm or 530/30 nm filter was used for MESF FITC microsphere detection, and a 585/42 or 590/20 nm filter for MESF PE microsphere detection. Loss of dim microsphere resolution is marked with black arrows.