Table 2. PE and red fluorescent proteins on a jet-in-air cell sorter.

Sensitivity index values are shown for BD FACSVantage DiVa™ analysis EL4 mouse thymoma cells were labeled with biotin-anti-CD3, CD44 or CD95 antibody followed by streptavidin conjugated to PE, and SP2/0 cells were stably transfected with DsRed and dTomato. Cells were analyzed on a using a 532 nm laser at 150 mW, and the 550 nm laser at 50, 100 and 150 mW. All cells were analyzed through the same 585/40 nm bandpass filter shown in Figure 4. PMT voltages were fixed for all samples.

		Sensitivity indices
		EL4 cells labeled with PE-conjugated			SP2/0 cells expressing
Laser wavelength (nm)	Laser power (mW)	CD3	CD44	CD90	DsRed	dTomato
550 nm	50	12.4	108.5	18.6	45.2	102.8
	100	12.2	102.7	17.1	59.0	133.5
	150	10.2	80.6	12.7	63.6	143.1
532 nm	150	9.87	82.7	15.1	27.6	58.5