Fig. 1. Toxoplasma 583.m00636 is RON5, which is processed into N and C-terminal fragments that are secreted into the moving junction.

A. Schematic of 583.m00636. The predicted signal peptide is shown in blue, and green brackets highlight residues 503–983 used to generate N-terminal antisera, and residues 1333–1675 to generate C-terminal antisera. B. Extracellular Toxoplasma lysate probed with 583.m00636 N and C-terminal antisera reveals two separate fragments migrating at ~110 and ~45 kDa. C. 583.m00636 N and C-terminal antisera used in immunofluorescence of intracellular Toxoplasma tachyzoites (1^st, 3^rd panels) or partially invaded Toxoplasma (2^nd, 4^th panels) show colocalization with Toxoplasma RON4 in both the rhoptry neck and the moving junction (arrows).