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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Cell Microbiol. 2008 Dec 30;11(4):590–603. doi: 10.1111/j.1462-5822.2008.01276.x

Fig. 2. Monoclonal antibody 8E3 detects a novel Neospora moving junction RON protein.

Fig. 2

A. IFA of intracellular Neospora parasites shows colocalization of 8E3 with Neospora RON4 (using Toxoplasma cross-reactive polyclonal RON4 antisera). B. 8E3 antibody staining colocalizes with RON4 in partially invaded parasites in both the rhoptry neck and the Neospora MJ (arrows). C. Western blot analysis of Neospora lysate reveals the protein detected by 8E3 is >250 kDa, considerably larger than the MJ/RONs 2, 4, and 5.

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