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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Cell Microbiol. 2008 Dec 30;11(4):590–603. doi: 10.1111/j.1462-5822.2008.01276.x

Fig. 7. The RON8 C-terminus is necessary and sufficient for trafficking and cytoskeletal colocalization.

Fig. 7

A. Schematic of RON8 deletion series involving successive removal of 947, 1618, and 2283 residues from the C-terminus of RON8-HA, while remaining in frame with the HA epitope tag. B. HEK293 cells transiently transfected with each deletion construct were stained with α-HA and phalloidin; all three constructs lack membrane/cytoskeleton targeting seen with full length RON8-HA expressing HEK293 cells. C. Diagram depicting residues 2348–2980 of RON8 fused to an HA tag, used for assessing targeting sufficiency. D. Transfection of this C-terminal region into either HEK293 or HeLa cells produces colocalization of RON8 (2348–2980) with the cortical cytoskeleton by IFA with α-HA sera and phalloidin costain.

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