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In vitro stability of conventional (pGEN222 and pGEN206) and SSB-maintained (pGEN222S2 and pGEN206S2) plasmids expressing GFPuv in CVD908-htrA and CVD908-htrAssb live vectors, respectively. Live vector strains were passaged at 37°C from an overnight starter culture (grown without selection) every 24 h for 5 days (120 h) in antibiotic-free liquid medium. Plasmid stability is reported as the percentage of fluorescing colonies compared to the total CFU count plated on nonselective medium.
